Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization
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چکیده
منابع مشابه
Suppression Subtractive Hybridization in Penaeid Prawns: An Approach in Identifying Diseases and Differentially Expressed Genes
Alteration in gene transcripts is related to a wide spectrum of physiological and pathological changes in cells. Therefore, the identification of differential gene expression can be used to understand biological processes at a molecular level [1-4]. Various techniques such as Expressed Sequence Tags (EST), differential display, Serial Analysis of Gene Expression (SAGE), cDNA microarray, and sub...
متن کاملSuppression subtractive hybridization.
Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...
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The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identified, of which 100 were down-regulated, and...
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The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes...
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Cellular senescence marks the end of the proliferative life span of normal cells in tissue culture and occurs after cells have undergone a certain number of population doublings (PDLs). It is accompanied by alterations in the pattern of gene expression. A specific human embryonic lung diploid fibroblast cell line, 2BS, has been studied as a model of senescence in our laboratory. Here, we report...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2009
ISSN: 1471-2164
DOI: 10.1186/1471-2164-10-206